DETECTION OF CAMPYLOBACTER USING STANDARD CULTURE AND PCR OF 16S rRNA GENE IN FRESHLY CHILLED POULTRY AND POULTRY PRODUCTS IN A SLAUGHTERHOUSE
نویسنده
چکیده
The present study provides data on contamination level with Campylobacter in poultry and poultry products after air chilling. Using cell culture and PCR we detected Campylobacter in the following proportion of chicken samples studied: 38.3% and 40.8%, respectively. Campylobacter contamination occurred highest in liver samples (53.3%), followed by the skin (46.6%), tight (36.6%) and breast (16.6%) samples in that order. C. jejuni (65.2%) was the most frequently isolated species, whereas C. coli, C. fetus and C. upsaliensis were isolated in 30.4%, 2.2%, and 2.2%., respectively. C. jejuni ssp. doylei (60%) was most commonly found subspecies of C. jejuni.
منابع مشابه
Isolation and identification of Campylobacter jejuni from poultry carcasses using conventional culture methods and multiplex PCR assay
Campylobacter jejuni is a major cause of food-borne diarrhea in many countries. Poultry and poultry products are known as important sources of human campylobacteriosis. In this study, conventional culture and multiplex PCR methods were compared for the detection of C. jejuni isolated from poultry carcasses. A total of 100 samples, representing 20 broiler flocks, were collected from poultry carc...
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